Undergraduate Research Assistant
Miller Lab member KC Kent is surveying providers who order genetic testing about their experience with long-read sequencing and their opinions about LRS as a clinical diagnostic test. No experience with LRS? That's OK – we still want to learn what factors you use to choose a genetic test. All participation is valuable.COMPLETE THE SURVEY
Very excited for Miller Lab member Sophie Gibson, who has been awarded an NIH/NHGRI Genome Training Grant through the UW Department of Genome Sciences.
Excited to receive the UW Department of Laboratory Medicine & Pathology's Building Bridges Award with co-PI Dr. Brian Shirts. This will help fund work by grad student Nikhita Damaraju, who is pursuing a PhD in Public Health Genetics.
Very happy to welcome two talented graduate students to our team, Sophie Gibson (Genome Sciences) and J. (Gus) Gustafson (Molecular & Cellular Biology).
Congratulations to Danny on being selected as one of this year's recipients of the American Society for Clinical Investigation's Young Physician-Scientist Award, which recognizes early-career physician-scientists who have made notable achievements in their research.
Danny here... Incredibly honored to be selected for an NIH Director’s Early Independence Award (DP5), and incredibly grateful to the mentors who helped me get here.
We did a thing! Using ONT long-read sequencing, we determined a newborn's genetic risk within three hours of the infant's birth. Check out the preprint.
READ IT! »
We're currently recruiting for a postdoc position. Ideal candidates should have programming knowledge as well as wet lab experience and an interest in long-read sequencing.APPLY HERE »
Nice writeup on GenomeWeb about our project to sequence 500 of the 1000 Genomes Project samples using Nanopore. Excited to work on this with Evan Eichler and about a hundred other folks!
Lots of green!
Hi folks, we're opening our lab in July 2022 in the Departments of Pediatrics and Laboratory Medicine & Pathology at the University of Washington. We use long-read sequencing on the Oxford Nanopore platform to find the genetic basis for rare genetic disorders. If you have interesting unsolved cases, drop us a note. We love collaborating on tricky problems!
We are interested in understanding why 50% of individuals with a suspected genetic disorder remain unsolved after a complete clinical evaluation. Using long-read DNA and RNA sequencing, we identify disease-causing variants that would be difficult or impossible to resolve using standard clinical testing. We are always happy to collaborate, so reach out if you have a challenging unsolved case.
Standard clinical genetic testing can take years to complete and is diagnostic only 50% of the time. It's a challenging process often referred to as the diagnostic odyssey. We are working to change this paradigm by using long-read sequencing as a single test in the clinical setting. Our efforts extend beyond the genetics clinic into spaces such as cancer biology and infectious disease.
A major challenge when analyzing long-read sequencing data is interpreting all of the structural variants that are found. In collaboration with Oxford Nanopore, we are sequencing a large number of 1000 Genomes Project samples to understand what normal human structural variation looks like and to build a database of controls for everyone to use. Check out the 1000G ONT Sequencing Consortium site for more information.
LEARN MORE »
Because long-read sequencing data contain signal for both the DNA sequence and methylation status, we can identify differences in methylation in unsolved cases and healthy controls. We are building tools to identify differences in methylation genome-wide and understand what methylation looks like in a large number of healthy controls.
This is how we got into Nanopore sequencing! We are always interested in sequencing and assembling genomes from all critters big and small. We're part of a large group working on sequencing all of the species in the Drosophila species group, and we're interested in all types of genome assembly projects.
Sequencing of native RNA is just cool. We do RNA sequencing of our unsolved clinical cases and are interested in using long reads to identify tissue-specific isoforms and expression. Also, what are all those RNA modifications doing? Sequencing of native RNA from different tissues is going to be interesting.
The Miller Lab operates the University of Washington Nanopore Sequencing Core, which provides long-read sequencing on the Oxford Nanopore Technologies (ONT) platform to researchers at UW, SCH, SCRI, FHCC, external institutions, and private companies. Services include whole-genome sequencing, RNA sequencing, variant calling and phasing, and methylation calling. If you are interested in long-read sequencing services, please visit the UW Nanopore Sequencing Core site for more information about sample preparation, options, and costs.
Research in the Miller Lab is a healthy mix of computational and bench science. We believe that training the next generation of scientists – at all levels – is one of our most important responsibilities. While we value hard work, we also know work–life balance matters, so if you'd like to be part of a respectful, collaborative, and productive team, we'd love to hear from you! Feel free to email with any inquiries.
We're currently recruiting for a postdoc position. To apply, please provide a current CV, a summary of your research accomplishments/goals/interests, and contact info for at least three references. Ideal candidates will have programming knowledge as well as wet lab experience and an interest in long-read sequencing. Click here for more information.
We are grateful for the generous support our research program receives from the National Institutes of Health Director's Early Independence Award, the UW Department of Laboratory Medicine & Pathology, and the Brotman Baty Institute for Precision Medicine.
We appreciate your interest in our research. If you would like to make a contribution to a Miller Lab study, please write a brief letter designating the funds to research by Danny E. Miller, MD, PhD, and indicate the study topic you wish to support. Include your name and address in the letter, and send it with a check or money order to:
Miller Laboratory, Department of Pediatrics
University of Washington
1959 NE Pacific Street, Box 356320
Seattle, WA 98195-6320