Around 50% of individuals with a suspected genetic disorder remain undiagnosed after a complete clinical evaluation, which often takes years to complete. We believe this burden on patients and families is simply too high. In the Miller Lab, our goals are twofold: to improve the efficiency and effectiveness of genetic testing, and to better understand human genetic disease through the identification and characterization of novel disease-causing variation.
Thanks to the Brotman Baty Institute for this nice writeup about some of the work we do in the Miller Lab. We're starting to test out single-cell Nanoopore sequencing, so let us know if you are interested in trying it out.LEARN MORE
Check out these recent talks at the 2022 Nanopore Community Meeting and a UW Laboratory Medicine & Pathology Grand Rounds to hear about how the Miller Lab is using long-read sequencing in unsolved genetic disorders.
Danny here... Incredibly honored to be selected for an NIH Director’s Early Independence Award (DP5), and incredibly grateful to the mentors who helped me get here.
We did a thing! Using ONT long-read sequencing, we determined a newborn's genetic risk within three hours of the infant's birth. Check out the preprint.
READ IT! »
We're currently recruiting for a postdoc position. Ideal candidates should have programming knowledge as well as wet lab experience and an interest in long-read sequencing.APPLY HERE »
Nice writeup on GenomeWeb about our project to sequence 500 of the 1000 Genomes Project samples using Nanopore. Excited to work on this with Evan Eichler and about a hundred other folks!
Lots of green!
We're excited to collaborate with ONT to sequence 500 of the 1000 Genomes samples. If you're interested in working on this project or joining the 1000G ONT Sequencing Consortium, please reach out.LEARN MORE »
Hi folks, we're opening our lab in July 2022 in the Departments of Pediatrics and Laboratory Medicine & Pathology at the University of Washington. We use long-read sequencing on the Oxford Nanopore platform to find the genetic basis for rare genetic disorders. If you have interesting unsolved cases, drop us a note. We love collaborating on tricky problems!
Danny's contribution to the Telomere-to-Telomere project was highlighted by the Brotman Baty Institute.LEARN MORE »
We're sequencing cases that will be presented during the Unsolved session at the 2022 David W. Smith Workshop this August. If you're attending the meeting and have an unsolved genetics case, let us know!
Danny's targeted long-read sequencing paper was highlighted in AJMG, GenomeWeb, and ClinicalOMICS!READ THE PAPER »
We are interested in understanding why 50% of individuals with a suspected genetic disorder remain unsolved after a complete clinical evaluation. Using long-read DNA and RNA seqeuencing, we have identified disease-causing variants that are difficult or impossible to resolve using standard clinical testing. We are always happy to collaborate, so reach out if you have a challenging unsolved case.
Standard clinical genetic testing can take years to complete and is diagnostic only 50% of the time. It's a challenging process often referred to as the diagnostic odyssey. We are working to change this paradigm by using long-read sequencing as a single test in the clinical setting. Our efforts extend beyond the genetics clinic into spaces such as cancer biology and infectious disease.
A big challenge when analyzing long-read sequencing data is interperting all of the structural variants that are found. In collaboration with Oxford Nanopore, we are seqeuencing a large number of 1000G Project samples to understand what normal human structural variation looks like and to build a database of controls for everyone to use. Check out the 1000G ONT Consortium site for more information.
LEARN MORE »
Because long-read seqeuncing data contain signal for both the DNA sequence and methylation status, we can identify differences in methylation in unsolved cases and healthy controls. We are building tools to identify differences in methylation genome-wide and understand what methylation looks like in a large number of healthy controls.
This is how we got into Nanopore sequencing! We are always interested in sequencing and assembling genomes from all critters big and small. We're part of a large group working on sequencing all of the species in the Drosophila species group, and we're interested in all types of genome assembly projects.
Sequencing of native RNA is just cool. We do RNA seqeuncing of our unsolved clinical cases and are interested in using long reads to identify tissue-specific isoforms and expression. Also, what are all those RNA modifications doing? Sequencing of native RNA from different tissues is going to be interesting.
The Miller Lab operates the University of Washington Nanopore Sequencing Core, which provides long-read sequencing on the Oxford Nanopore Technologies (ONT) platform to researchers at UW, SCH, SCRI, FHCC, external institutions, and private companies. Services include whole-genome sequencing, RNA sequencing, variant calling and phasing, and methylation calling. If you are interested in long-read sequencing services, please visit the UW Nanopore Sequencing Core site or email us for more information about options and costs.
Research in the Miller Lab is a healthy mix of computational and bench science. We believe that training the next generation of scientists – at all levels – is one of our most important responsibilities. While we value hard work, we also know work–life balance matters, so if you'd like to be part of a respectful, collaborative, and productive team, we'd love to hear from you! Feel free to email with any inquiries.
We're currently recruiting for a postdoc position. To apply, please provide a current CV, a summary of your research accomplishments/goals/interests, and contact info for at least three references. Ideal candidates will have programming knowledge as well as wet lab experience and an interest in long-read sequencing. Click here for more information.
This position primarily involves preparing samples for DNA and RNA sequencing on the Oxford Nanopore platform. This is a great opportunity for an organized individual with basic laboratory skills.
Grad students who want to learn more about long-read DNA or RNA sequencing, structural variation, genome assembly, or developing long-read-sequencing-based clinical tests are encouraged to rotate in our lab. We are currently able to accept MCB and M3D grad students, and we're hoping to include other UW programs soon. Stay tuned!
We are now accepting applications for summer undergrads. To apply for an undergraduate position, please submit your current CV, including any relevant experience or courses you've taken, as well as a couple paragraphs about why you want to do research and why in our lab specifically.
We are grateful for the generous support our research program receives from the National Institutes of Health Early Independence Award as well as private foundation support from the Brotman Baty Institute for Precision Medicine.
We appreciate your interest in our research. If you would like to make a contribution to a Miller Lab study, please write a brief letter designating the funds to research by Danny E. Miller, MD, PhD, and indicate the study topic you wish to support. Include your name and address in the letter, and send it with a check or money order to:
Miller Laboratory, Department of Pediatrics
University of Washington
1959 NE Pacific Street, Box 356320
Seattle, WA 98195-6320