We did a thing! Using ONT long-read sequencing, we determined a newborn's genetic risk within three hours of the infant's birth. Check out the preprint.
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We're currently recruiting for a postdoc position. Ideal candidates should have programming knowledge as well as wet lab experience and an interest in long-read sequencing.APPLY HERE »
Nice writeup on GenomeWeb about our project to sequence 500 of the 1000 Genomes Project samples using Nanopore. Excited to work on this with Evan Eichler and about a hundred other folks!
Lots of green!
Hi folks, we're opening our lab in July 2022 in the Departments of Pediatrics and Laboratory Medicine & Pathology at the University of Washington. We use long-read sequencing on the Oxford Nanopore platform to find the genetic basis for rare genetic disorders. If you have interesting unsolved cases, drop us a note. We love collaborating on tricky problems!
We are interested in understanding why 50% of individuals with a suspected genetic disorder remain unsolved after a complete clinical evaluation. Using long-read DNA and RNA seqeuencing we have identified disease-causing variants that are difficult or impossible to resolve using standard clinical testing. We are always happy to collaborate, so reach out if you have a challenging unsolved case.
Standard clinical genetic testing can take years to complete and is diagnostic only 50% of the time. It's a challenging process often referred to as the diagnostic odyssey. We are working to change this paradigm by using long-read sequencing as a single test in the clinical setting. Our efforts extend beyond the genetics clinic into spaces such as cancer biology and infectious disease.
A big challenge when analyzing long-read sequencing data is interperting all of the structural variants that are found. In collaboration with Oxford Nanopore, we are seqeuencing a large number of 1000G Project samples to understand what normal human structural variation looks like and to build a database of controls for everyone to use. Check out the 1000G ONT Consortium site for more information.
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Because long-read seqeuncing data contain signal for both the DNA sequence and methylation status, we can identify differences in methylation in unsolved cases and healthy controls. We are building tools to identify differences in methylation genome-wide and understand what methylation looks like in a large number of healthy controls.
This is how we got into Nanopore sequencing! We are always interested in sequencing and assembling genomes from all critters big and small. We're part of a large group working on sequencing all of the species in the Drosophila species group, and we're interested in all types of genome assembly projects.
Sequencing of native RNA is just cool. We do RNA seqeuncing of our unsolved clinical cases and are interested in using long reads to identify tissue-specific isoforms and expression. Also, what are all those RNA modifications doing? Sequencing of native RNA from different tissues is going to be interesting.
We provide long-read sequencing on the Oxford Nanopore Technologies (ONT) platform to researchers at UW, SCH, SCRI, FHRC, external institutions, and private companies. Services include whole-genome sequencing, targeted sequencing, RNA sequencing, variant calling and phasing, and methylation calling. If you are interested in long-read sequencing services, please contact us to discuss options and costs.
We have several available positions in the Miller Lab. Although our lab is heavily computational, benchwork is also necessary. You'll be joining a friendly group of people with diverse backgrounds. Danny (a runner, and a former programmer and financial analyst) is a Pediatrician and Medical Geneticist with extensive experience analyzing long-read ONT data. If you're looking for a fun, collaborative, and productive work environment, we'd love to hear from you! Please email with any inquiries.
We're currently recruiting for a postdoc position. Ideal candidates should have programming knowledge as well as wet lab experience and an interest in long-read sequencing. Click below for more information.
Mentoring students is one of our most important responsibilities. Grad students who want to learn more about long-read DNA or RNA sequencing, structural variation, genome assembly, or developing long-read-sequencing-based clinical tests are encouraged to rotate in our lab.
We also welcome undergraduate students in our lab. Please reach out if you're interested!
We are grateful for the generous support our research program receives from the Brotman Baty Institute for Precision Medicine.
We appreciate your interest in our research. If you would like to make a contribution to a Miller Lab study, please write a brief letter designating the funds to research by Danny E. Miller, MD, PhD, and indicate the name of the study you wish to support. Include your name and address in the letter, and send it with a check or money order to:
Miller Laboratory, Department of Pediatrics
University of Washington
1959 NE Pacific Street, Box 356320
Seattle, WA 98195-6320